Quorum Quenching Bacillus sonorensis Isolated from Soya Sauce Fermentation Brine
نویسندگان
چکیده
An N-acylhomoserine lactone (AHL)-degrading bacterial strain, L62, was isolated from a sample of fermentation brine of Chinese soya sauce by using rich medium agar supplemented with soya sauce (10% v/v). L62, a rod-shaped Gram positive bacterium with amylolytic activity, was phylogentically related to Bacillus sonorensis by 16S ribosomal DNA and rpoB sequence analyses. B. sonorensis L62 efficiently degraded N-3-oxohexanoyl homoserine lactone and N-octanoylhomoserine lactone. However, the aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of AHLs, was not detected in L62, suggesting the presence of a different AHL-degrading gene in L62. To the best of our knowledge, this is the first report of AHL-degrading B. sonorensis from soya sauce liquid state fermentation.
منابع مشابه
Full Paper Phylogeny of γ-polyglutamic acid-producing Bacillus strains isolated from a fermented locust bean product manufactured in West Africa
Japanese commercial natto, a sticky fermented food made from steamed soybeans, is produced using a pure culture of Bacillus subtilis, but natto-like products in other Asian countries are manufactured through a natural fermentation process (Chantawannakul et al., 2002; Chunhachart et al., 2006b; Sarkar et al., 2002; Tamang, 1999). These fermented soybean foods are different from brine-added ferm...
متن کاملComparison between traditional and industrial soy sauce (kecap) fermentation in Indonesia
Growth of lactic acid bacteria and amino acid production at an Indonesian soy sauce manufacturer, employing modern Japanese process technology, indicated that brine fermentation for one month is sufficient for industrial kecap production. Compared to traditional Indonesian soy sauce fermentation, application of modern Japanese process technology resulted in an obvious but not essential yeast fe...
متن کاملMicrobial succession and the functional potential during the fermentation of Chinese soy sauce brine
The quality of traditional Chinese soy sauce is determined by microbial communities and their inter-related metabolic roles in the fermentation tank. In this study, traditional Chinese soy sauce brine samples were obtained periodically to monitor the transitions of the microbial population and functional properties during the 6 months of fermentation process. Whole genome shotgun method reveale...
متن کاملRapid isolation method of soil bacilli and screening of their quorum quenching activity
In Gram-negative bacteria, quorum sensing is mediated by N-acylhomoserine lactones (AHL) to regulate different biological functions, including production of virulence factors. Quorum quenching refers to the interruption of quorum sensing, and the most attractive way is to degrade the AHL molecules. With the aim of isolating soil bacteria capable of blocking quorum sensing by inactivating AHL, a...
متن کاملProducing fish sauce from Caspian kilka
Fish sauce is a fermented product which is used in south Asian countries. In the present study, Caspian Kilka was used to produce the sauce, using either cooked or raw fish subjected to four different treatments: 1) traditional method, where fish and salt were used; 2) an enzymatic method, where fish, salt and proteolytic enzymes, including Protamex and Flavourzyme (Novo Nordisc Co., Bagsvaerd,...
متن کامل